Journal: Arthritis Research & Therapy
Article Title: IL-35 enhances IL-10⁺ breg-mediated immunoregulation and attenuates inflammation and fibrosis in systemic sclerosis
doi: 10.1186/s13075-026-03735-8
Figure Lengend Snippet: Expression levels of B cell subsets and related cytokines in peripheral blood of systemic sclerosis patients and healthy controls. A Flow cytometry gating strategy and staining patterns showing frequencies of B cells, interleukin-10+ regulatory B cells (IL-10+Breg), IL-35+Breg cells, and IL-6+ effector B cells (IL-6+Beff). B Plasma concentrations of IL-35, IL-10, B cell activating factor (BAFF), and IL-6. C Relative mRNA expression of IL-10, IL-6, and transforming growth factor-β1 (TGF-β1) in skin tissue. D Immunofluorescence images of IL-10+Breg cells in skin tissue and their proportion among CD19+B cells (scale bar: 50 μm, 400× magnification). E Immunofluorescence images of IL-35+Breg cells in skin tissue and their proportion among CD19+B cells (scale bar: 50 μm, 400× magnification). F Heatmap showing correlations between circulating B cell subsets/cytokines and clinical parameters. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns, not significant
Article Snippet: The following reagents were used: Rat polyclonal CD19 antibody (Columbia, MD, USA; Cat# 32727), a Mouse IL-6 antibody (Santa Cruz Biotechnology, USA; Cat# SC-28343), Mouse monoclonal CD19 antibody (Novus Biologicals, USA; Cat# NBP2-25196SS), Rat Anti-IL-10 antibody (Abcam, Cambridge, UK; Cat# ab133575), and Rat IL-35 Antibody (Abbexa, Cambridge, UK; Cat# Abx274658),
Techniques: Expressing, Flow Cytometry, Staining, Clinical Proteomics, Immunofluorescence